#Created with Version: 1158 #Sequence length L_s. L_s[bases]=24 #Length of basic sequences (criton length) 1 <= L_c <= 13. L_c[bases]=5 #Forbidden stem length of hairpin structures L_hp (blank for any size). L_hp[base pairs]=3 #Length of forbidden self-complementary subsequences L_sc (blank for any size). L_sc[bases]=4 #Minimum number of G/C-bases per strand GC_min, minimum GC content (blank for any number). GC_min[bases]=11 #Maximum number of G/C-bases per strand GC_max, maximum GC content (blank for any number). GC_max[bases]=13 #Length of subsequences that are not allowed to be repeated within one and the same sequence (sliding) L_sl (blank for any size). L_sl[bases]=5 #No terminal adenine or thymine in the strand?/Demand on GC ends? [y/n] GC_ends=n #Maximum number of sequences to be generated per set. SeqMax=1250 #Maximum number of sets to be generated. SetMax=2 #Maximum number of attempts to restart the generation of one sequence. MaxAttempt=50 #Termination factor to limit the number of combination attempts with basic sequences for the generation of one strand. TerminationFactor=1 #Name of the file with sequences to be included (blank, if no file exist). Included= #Name of the file with neighboring sequences (blank, if no file exist). Neighboring= #Forbidden stem length of hairpin structures with neighboring sequences L_ni (neighbor interaction). L_ni[base pairs]= #Calculate deltaG? [y/n] deltaG=n